A novel function for TPX2 1 Targeting Protein for Xenopus kinesin like protein 2 (TPX2) regulates gamma-H2AX levels upon ionizing radiation
نویسندگان
چکیده
*Departments of Clinical Neurosciences and Cell Biology & Anatomy, Hotchkiss Brain Institute, ◊Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Drive NW, T2N4N1, Canada. ♦ Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S-171 21 Stockholm, Sweden. ^Leiden University Medical Center, Department of Toxicogenetics, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands. # DKFZ-ZMBH Alliance, ZMBH, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany. ~ Equal contribution.
منابع مشابه
Targeting protein for xenopus kinesin-like protein 2 (TPX2) regulates γ-histone 2AX (γ-H2AX) levels upon ionizing radiation.
The microtubule-associated protein targeting protein for Xenopus kinesin-like protein 2 (TPX2) plays a key role in spindle assembly and is required for mitosis in human cells. In interphase, TPX2 is actively imported into the nucleus to prevent its premature activity in microtubule organization. To date, no function has been assigned to nuclear TPX2. We now report that TPX2 plays a role in the ...
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During interphase, the spindle assembly factor TPX2 is compartmentalized in the nucleus where its roles remain largely uncharacterized. Recently, we found that TPX2 regulates the levels of serine 139-phosphoryated H2AX (γ-H2AX) at chromosomal breaks induced by ionizing radiation. Here, we report that TPX2 readily associates with the chromatin in the absence of ionizing radiation. Overexpression...
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TPX2, the targeting protein for Xenopus kinesin-like protein 2 (Xklp2), was identified as a microtubule-associated protein that mediates the binding of the COOH-terminal domain of Xklp2 to microtubules (Wittmann, T., H. Boleti, C. Antony, E. Karsenti, and I. Vernos. 1998. J. Cell Biol. 143:673-685). Here, we report the cloning and functional characterization of Xenopus TPX2. TPX2 is a novel, ba...
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In humans, the targeting protein for Xenopus kinesin-like protein 2 (TPX2) is a cell cycle-associated protein, and altered TPX2 expression has been found in various malignancies. However, the contribution of TPX2 expression to astrocytoma progression is unclear. The aim of this study was to investigate TPX2 expression in human astrocytoma samples and cell lines. TPX2 protein expression was dete...
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